Anti-TNFβ/LTA Antibody, clone 3B20-H2 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 3B20-H2 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects TNFβ/LTA.
• Immunogen: KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human TNFβ (Lymphotoxin-a).
• Application: Quality Control TestingEvaluated by Western Blotting with human recombinant TNFβ/LTA.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human TNFβ/LTA. Tested applicationsFlow Cytometry Analysis: 1 mg from a representative lot detected TNFβ/LTA in one million HepG2 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TNFβ/LTA in human tonsil tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Lymphotoxin-a (UniProt: P01374; also known as LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1) is encoded by the LTA (also known as TNFB, TNFSF1) gene (Gene ID: 4049) in human. Lymphotoxin-a is cytokine of the tumor necrosis family that is synthesized by lymphocytes with a signal peptide (aa 1-34), which is subsequently cleaved off to generate the mature form. It is shown to be cytotoxic for a wide range of tumor cells in vitro and in vivo. It can exist as a secreted homotrimeric form or can form a heterotrimer with lymphotoxin-a to become a membrane associated form. The homotrimeric form is shown to bind to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM and the heterotrimer binds exclusively to TNFRSF3/Lymphotoxin- receptor (LTBR). Lymphotoxin- is reported to be involved in the formation of secondary lymphoid organs during development and play a role in apoptosis. Mutations in LTA gene are known to cause Psoriatic arthritis, an inflammatory seronegative arthritis, which is a heterogeneous disorder that can range from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.