Anti-GST Antibody, clone 5B8 , ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 5B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glutathione S Transferase.
• Immunogen: Full-length recombinant E. Coli Glutathione S-transferase.
• Application: Anti-GST, clone 5B8 ZooMAb, Cat. No. ZRB1223, is a recombinant Rabbit monoclonal antibody that specifically detects Glutathione S Transferase and has been tested for use in Dot Blot, ELISA, Immunocytochemistry and Western Blotting.
• Target description: Glutathione S-transferase (also known as GST) is encoded by the Gst gene in E. coli. Glutathione S transferases are a group of enzymes that are responsible for the detoxification of reactive chemical species through conjugation to reduced glutathione. They are ubiquitously distributed in nature, being found in organisms from microbes to higher mammals. They exist as membrane-bound as well as cytosolic enzymes with distinct catalytic and noncatalytic binding properties. They catalyze the conjugation of reduced glutathione (GSH) with compounds that contain an electrophilic center through the formation of a thioether bond between the sulfur atom of GSH and the substrate. The fundamental basis of their catalytic activity has been attributed to their capacity to lower the pKa of the sulfhydryl group of GSH from 9.0 in aqueous solution to about 6.5 when GSH is bound in the active site. In the case of certain substrates, GST can catalyze both the forward and reverse reactions, which can lead to increased toxicity rather than detoxication. GST has also been used as a tag for recombinant proteins where it can be fused with either the N- or the C-terminal of protein. Proteins produced using the GST fusion system have been used in numerous biological applications. It allows proteins to become more soluble. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Nebert, DW., and Vasiliou, V. (2004). Hum. Genomics. 1(6); 460-464).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.