Anti-HDAC1 Antibody, clone 4D21 , ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 4D21 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Histone Deacetylase 1. It targets an epitope within 17 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Histone Deacetylase 1.
• Application: Anti-HDAC1, clone 4D21 ZooMAb, Cat. No. ZRB1231, is a recombinant Rabbit monoclonal antibody that specifically targets Histone deacetylase 1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Histone deacetylase 1 (UniProt: Q13547; also known as EC:3.5.1.98, HD1, HDAC1) is encoded by the HDAC1 (also known as RPD3L1) gene (Gene ID: 3065) in human. HDAC1, a member of Class I HDACs is widely expressed in tissues and is primarily located in the nucleus. Higher expression of HDAC1 is observed in heart, pancreas, and testis. It catalyzes the removal of the acetyl moiety from the -amino groups of lysine residues of histones to yield deacetylated histones H2A, H2B, H3 and H4, which results in compaction of chromatin into a transcriptionally repressed state. HDAC1 is a component of the BRG1-RB1-HDAC1 complex, which is reported to negatively regulate the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. HDAC1 is also shown to deacetylate lysine 310 in RelA and thereby inhibits the transcriptional activity of NF-kappa-B. HDAC1 undergoes sumolyation on lysine 444 and lysine 476, which promotes its enzymatic activity. In addition, phosphorylation on serine 421 and serine 423 by Cdk5 also promotes its activity and interactions with nucleosome remodeling and deacetylase (NuRD) and SIN3 complexes. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.