Anti-Caspase-1 (p20) Antibody, clone 2G4 , ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2G4 is a ZooMAb rabbit recombinant monoclonal antibody that detects Caspase-1 proenzyme and the p20 subunit. It targets an epitope within 21 amino acids from the C-terminal region of p20 subunit.
• Immunogen: KLH-conjugated linear peptide corresponding to 21 amino acids from the p20 subunit of human Caspase-1.
• Application: Anti-Caspase-1 (p20), clone 2G4 ZooMAb, Cat. No. ZRB1233, is a recombinant Rabbit monoclonal antibody that targets Caspase-1 proenzyme and p20 subunit and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Caspase-1 (UniProt: P29466; also known as EC:3.4.22.36, CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45) is encoded by the CASP1 (also known as IL1BC, IL1BCE) gene (Gene ID: 834) inn human. Caspse-1 is a thiol protease that is synthesized as a single chain zymogen, which is further processed to create active caspase-1. The zymogen has two propeptides (aa 1-119 and 298-316), that are subsequently cleaved off to generate p20 (aa 120-297) and p10 (aa 317-404) subunits. The mature enzyme consists of two anti-parallel arranged heterodimers, each formed by a p20 and a p10 subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. It displays a strict requirement for an aspartic acid residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp. Caspase-1 cleaves IL-1 beta between an aspartic acid and alanine, releasing the mature cytokine which is involved in a variety of inflammatory processes. It is an important component of the inflammasome that during DNA virus infection controls antiviral immunity through cleavage of cGMP-AMP synthase (cGAS), rendering it inactive. Caspase-1 is expressed in larger amounts in spleen and lung, but is also detected in most other tissues, except for the brain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, Y., et al. (2017). Immunity. 46(3); 393-404).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.