Anti-Cyclin D1 Antibody, clone 1J9, ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1J9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Cyclin D1. It targets an epitope within 19 amino acids from the C-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Cyclin D1.
• Application: Anti-Cyclin D1, clone 1J9 ZooMAb, Cat. No. ZRB1313, is a recombinant rabbit monoclonal antibody that specifically detects Cyclin D1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: G1/S-specific cyclin-D1 (UniProt: P24385; also known as B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene) is encoded by the CCND1 (also known as BCL1, PRAD1) gene (Gene ID: 595) in human. Cyclin D1 is an considered to be an essential regulator of the G1-S transition in response to growth factor stimulation in somatic cells. Together with cyclin dependent kinases 4 and 6 (CDK4 and CDK6), it forms active complexes that are shown to promote cell cycle progression by phosphorylating and inactivating the retinoblastoma protein (RB). Phosphorylation of RB1 allows dissociation of E2F from the RB/E2F complex and the subsequent transcription of E2F target genes that are responsible for the progression through the G1 phase. Cyclin D1 levels are reported to rise early in G1 and continue to accumulate until the G1/S-phase boundary when its levels rapidly decline. It exhibits a short half-life of about 24 minutes and is readily degraded in the 26S proteasome. Cyclin D1 is shown to repress DNA replication by binding to proliferating cell nuclear antigen (PCNA) and Cdk2. The binding of cyclin D1 to PCNA directly inhibits DNA synthesis. The overexpression of cyclin D1 and its deregulated degradation have been linked to the development and progression of various cancers. A chromosomal aberration involving CCND1 gene is reported to cause B-lymphocytic malignancy. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Alao, JP (2007). Mol. Cancer 6: 24; Albero, R., et al. (2018). J. Clin. Invest. 128(9); 4132-4147).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.