Anti-PIAS Antibody, clone 1O17 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1O17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects E3 SUMO-protein ligase PIAS1. It targets an epitope within the C-terminal region.
• Immunogen: GST/His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal region of human E3 SUMO-protein ligase PIAS1 protein.
• Application: Anti-PIAS, clone 1O17 ZooMAb, Cat. No. ZRB1450, is a recombinant Rabbit monoclonal antibody that specifically targets PIAS and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: E3 SUMO-protein ligase PIAS1 (UniProt: O75925; also known as DEAD/H box-binding protein 1, E3 SUMO-protein transferase PIAS1, Gu-binding protein, GBP, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein) is encoded by the PIAS1 (also known as DDXBP1) gene (Gene ID: 8554) in human. PIAS is an E3-type small ubiquitin-like modifier (SUMO) ligase that is expressed in numerous tissues with highest levels found in the testis. It stabilizes the interaction between UBE2I and the substrate and also acts as a SUMO-tethering factor. PIAS plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. It is known to enhance the sumoylation of MTA1 and may also participate in its paralog-selective sumoylation. It also plays a role in adipogenesis by promoting the SUMOylation and degradation of CEBPB. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.