Anti-Cytokeratin-14 Antibody, clone 1D15 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1D15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects cytokeratin-14. It targets an epitope within 15 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Cytokeratin-14.
• Application: Anti-Cytokeratin-14, clone 1D15 ZooMAb, Cat. No. ZRB1441, is a recombinant Rabbit Monoclonal antibody that targets Cytokeratin-14 and is tested in Immunocytochemistry and Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Keratin, type I cytoskeletal 14 (UniProt: P02533; also known as Cytokeratin-14, CK-14, Keratin-14, K14) is encoded by the KRT14 gene (Gene ID: 3861) in human. The keratins are the intermediate filament proteins of epithelia that display high degree of molecular diversity. They are heteropolymeric filaments formed by pairing of type I and type II keratins that are expressed in a highly specific patterns depending on the epithelial type and stage of cellular differentiation. Keratins contain a central rod domain of about 310 amino acids with alpha-helical conformation bordered by non-helical head and tail domains of variable length. The head domain consists of subdomains V1 and H1. The central alpha helical rod domain is composed of subdomains 1A, 1B, 2A, and 2B connected by the linkers L1, L12, and L2. The tail domain then consists of subdomains H2 and V2. They provide stability between epithelial cells and their attachment to basement membrane. Keratins also serve as tumor markers and epithelial differentiation markers. Cytokeratin-14 is detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum, but is not detected in stratum corneum. It is strongly expressed in the outer root sheath of anagen follicles. It is present in nucleus and cytoplasm and is expressed in a filamentous pattern. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.