Anti-RAD21 Antibody, clone 2H12 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2H12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects RAD21. It targets an epitope within 102 amino acids from the C-terminal half.
• Immunogen: GST/His-tagged recombinant fragment corresponding to 102 amino acids from the C-terminal half of human Double-strand-break repair protein Rad21 homolog (RAD21).
• Application: Anti-RAD21, clone 2H12 ZooMAb, Cat. No. ZRB1458, is a recombinant Rabbit monoclonal antibody that specifically targets RAD21 and is tested for used in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Double-strand-break repair protein rad21 homolog (UniProt: O60216; also known as hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog) is encoded by the RAD21 (also known as HR21, KIAA0078, NXP1, SCC1) gene (Gene ID: 5885) in human. RAD21 is a double-strand-break repair protein rad21 homolog. It is a component of the cohesion complex and is integral to chromosome segregation, error-free DNA repair, and prevention of inappropriate recombination between repetitive regions. It is cleaved by separase/ESPL1 at the onset of anaphase and this cleavage is required for sister chromatid separation and cytokinesis. The cohesin complex also plays a role in spindle pole assembly during mitosis. RAD21 expression in colorectal cancer is associated with aggressive disease especially in KRAS mutant tumors and resistance to chemoradiotherapy. Mutations in RAD21 gene have been linked to Cornelia de Lange syndrome that is characterized by facial dysmorphisms, abnormal hands and feet, growth delay and cognitive retardation. Some mutations can also cause Mungan syndrome that leads to neuromyopathy, chronic intestinal pseudo-obstruction and a variety of cardiac valve defects. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Deb, S., et al. (2014). Br. J. Cancer 110(6); 1606-1613).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.