Anti-Cyclin E Antibody, clone 1C7 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1C7 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Cyclin E. It targets an epitope within 21 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Cyclin E.
• Application: Quality Control TestingEvaluated by Western Blotting in K562 cell nuclear extract.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cyclin E in K562 cells nuclear extract.Tested ApplicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cyclin E in Human placenta tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cyclin E in K562 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Cyclin E (UniProt: P24864; also known as G1/S-specific cyclin-E1) is encoded by the CCNE1 (also known as CCNE) gene (Gene ID: 898) in human. Cyclin E is a nuclear protein that is essential for the control of the cell cycle at the G1/S transition. Its high expression is observed in testis and placenta and lower expression is observed in bronchial epithelial cells. The major role of cyclin E is to promote the assembly of the prereplication complex, through the loading of Cdc6 to the origins of replication and recruitment of the minichromosome maintenance protein complex (Mcm2-7), during the transition from quiescent G0 to G1 phases of the cell cycle and subsequently to trigger replication origin to allow cells to enter S phase. Cyclin E interacts with CDK2 to form a serine/threonine kinase holoenzyme complex where the cyclin subunit imparts substrate specificity to the complex. It serves a regulatory function and its binding to CDK2 activates the enzyme that can then phosphorylate severa
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.