Anti-DNA Polymerase beta Antibody, clone 3F21 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 3F21 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects DNA polymerase β. It targets an epitope within 13 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human DNA polymerase β.
• Application: Anti-DNA Polymerase β, clone 3F21 ZooMAb, Cat. No. ZRB1491, is a Rabbit monoclonal antibody that specifically targets DNA polymerase β and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: DNA polymerase beta (UniProt: P06746; also known as EC:2.7.7.7, DNAP, Polβ, DNA pol beta) is encoded by the POLB gene (Gene ID: 5423) in human. Pol β is a member of the X family of DNA polymerases. It plays a role in the base excision repair pathway that is essential to cleanse the genome of simple base lesions and abasic sites that arise in DNA from spontaneous base loss. Polβ is cytoplasmic under normal conditions, but it translocates to the nucleus following DNA damage where it associates with AP endonuclease 1 (Apex) to carry out DNA repair. It possesses two catalytic activities: DNA polymerase activity and dRP lyase activity. The active site for each of these activities is located on a distinct domain of the protein: 31 kDa polymerase domain and amino-terminal 8 kDa lyase domain. Polβ does not have proofreading activity; however, its accuracy in vitro results exclusively from the nucleotide selectivity of this enzyme. Aberrant Polβ overexpression results in an increased rate of spontaneous mutagenesis and a highly mutagenic tolerance phenotype and about thirty percent of all tumors are reported to harbor mutations in the POLB gene. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Beard WA., et al. (2006). Methods Enzymol. 408; 91-107).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.