Anti-FoxP3 Antibody, clone 4J2 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 4J2 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Forkhead box protein P3 (FoxP3). It targets an epitope within 14 amino acids from the C-terminal, the DNA binding domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal, DNA-binding region of human Forkhead box protein P3.
• Application: Anti-FoxP3, clone 4J2 ZooMAb, Cat. No. ZRB1565, is a recombinant Rabbit monoclonal antibody that specifically targets FoxP3 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
• Target description: Forkhead box protein P3 (UniProt: Q9BZS1; also known as Scurfin, FoxP3) I encoded by the FOXP3 (also known as IPEX, JM2) gene (Gene ID: 50943) in human. FoxP3 is homodimeric protein that Is predominantly expressed in the cytoplasm in activated conventional T-cells whereas it is mainly expressed in the nucleus in regulatory T-cells (Treg). It serves as a transcriptional regulator and is crucial for the development and inhibitory function of regulatory T-cells. The 41 kDa form derived by proteolytic processing is found exclusively in the chromatin fraction of activated Treg cells. Its levels are reported to be down-regulated in regulatory T-cells during inflammation. FoxP3 can undergo proteolytic cleavage in activated regulatory T-cells and can be cleaved at either the N- (aa 51-52) or C-terminal (aa 417-418) site, or at both sites. FoxP3 plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Its dimerization is reported to be essential for its transcriptional regulator activity. Is phosphorylation at serine 418 is shown to regulate its transcriptional repressor activity and consequently, regulatory T-cells suppressive function. FoxP3 is synthesized with a propeptide (aa 418-431), which is subsequently cleaved off in the mature form. Its nuclear localization signal is in the amino acids 414-417 and 68-76 and its nuclear export signal is localized in amino acids 239-249. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.