Anti-COX IV Antibody, clone 1L23 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1L23 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Cytochrome c oxidase subunit 4 isoform 1 (COX-IV). It targets an epitope within 20 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Cytochrome c oxidase subunit 4 (COX IV).
• Application: Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected COX IV in HeLa cell lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected COX IV in HepG2 and MCF-7 cell lysate.Flow Cytometry Analysis: 1 μg from a representative detected COX IV in one million HepG2 cells.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative detected COX IV in human heart tissue sections.Immunocytochemistry Analysis: A 1:100 Dilution from a representative detected COX IV in HeLa cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (UniProt: P13073; also known as Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1) is encoded by the COX4I1 (also known as COX4) gene (Gene ID: 1327) in human. COX IV is a single-pass membrane protein that is synthesized with a mitochondria transit peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-98), a transmembrane domain (99-124), and a cytoplasmic domain (aa 125-169). It is a component of the cytochrome c oxidase (complex IV), a multi-subunit enzyme composed of 14 subunits. Cytochrome c oxidase catalyzes the final step in mitochondrial electron transfer chain and is regarded as one of the major regulation sites for oxidative phosphorylation. It catalyzes the reduction of oxygen to water. The complex is composed of a catalytic core of 3 subunits MT-CO1, MT-CO2, and MT-CO3 and 11 supernumerary subunits COX4I1, COX5A, COX5B, COX6A1/2, COX6B1/2, COX6C, COX7A1/2, COX7B, COX7C, COX8A, and NDUFA4. The catalytic core subunits are encoded by mitochondrial DNA and the supernumerary subunits are encoded by the nuclear genome. Mutations in COX4 are known to cause nuclear type 16 mitochondrial complex IV deficiency, an autosomal recessive disorder that results in poor growth, microcephaly, hypotonia, and cerebral and cerebellar atrophy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, Y., et al. (2006). J. Bioenerg. Biomembr. 38(5-6); 283-291).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.