Anti-NSE/Enolase-2 Antibody, clone 2J18-H1 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2J18-H1 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Neuron-specific enolase (NSE/Enolase-2). It targets an epitope within 19 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Neuron-specific enolase (NSE/Enolase-2).
• Application: Quality Control TestingEvaluated by Western Blotting in Jurkat cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected NSE/Enolase-2 in Jurkat cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected NSE/Enolase-2 in lysates from C6 cells, Rat brain and Mouse tissue, and SHSY5Y cells.Affinity Binding Assay: A representative lot of this antibody bound NSE/Enolase-2 peptide with a KD of 4.5 x 10-9 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NSE/Enolase-2 in human cerebral cortex tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Gamma-enolase (UniProt: P09104; also known as EC:4.2.1.11, 2-phospho-D-glycerate hydrolyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE) is encoded by the ENO2 gene (Gene ID: 2026) in human. In vertebrates three isozymes of enolase have been described that are expressed by different genes. They are known as enolase , which is ubiquitous; enolase β is a muscle-specific form, and enolase is the neuron-specific form (NSE). NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. NSE exhibits neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It is expressed as - and as -dimer, is a late event in neural differentiation. Hence, it can serve as a useful index of neural maturation. Its levels in cord blood are useful in in confirming the diagnosis of neuroblastoma in newborns. It can also be used as a quantitative measures of brain damage following ischemic stroke, intracerebral hemorrhage, seizures etc. Increased NSE serum levels are also observed in various tumors. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Isgro, MA., et al. (2015). Adv. Exp. Med. Biol. 867:125-43).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.