Anti-pan-Na+ Channel Antibody, clone 4B8 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 4B8 is a ZooMAb® rabbit recombinant monoclonal antibody that detects various sodium channels. It targets an epitope within 18 amino acids from the 10th cytoplasmic domain in the C-terminal half. It reacts with all known Sodium channel (SCN) alpha subunits.
• Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the 10th cytoplasmic domain in the C-terminal region of human Sodium channel protein subunit alpha.
• Application: Quality Control TestingEvaluated by Western Blotting in Rat brain tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected pan-Na+ Channel in Rat brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected pan-Na+ Channel in human and mouse brain tissue lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected pan-Na+ Channel in E18 rat cortical cells.Affinity Binding Assay: A representative lot of this antibody bound Sodium Channel-Pan peptide with a KD of 1.0 x 10-12 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Sodium channel protein type 9 subunit alpha (UniProt: Q15858; also known as Neuroendocrine sodium channel, hNE-Na, Peripheral sodium channel 1, PN1, Sodium channel protein type IX subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.7, SCN9A) is encoded by the SCN9A (also known as NENA) gene (Gene ID: 6335) in human. Sodium channels are multi-pass membrane glycoproteins that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane sodium-selective channel allow sodium ions to pass in accordance with their electrochemical gradient. Nine different sodium ion channels have been described and they have been classified based on their sensitivity to tetrodotoxin. Mammalian sodium channels are heterotrimers that are composed of a large central pore-forming glycosylated alpha subunit and 2 smaller auxiliary beta subunits. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.