Anti-ERK5 Antibody, clone 2H15 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2H15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects ERK5. It targets an epitope within 18 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human ERK5.
• Application: Quality Control TestingEvaluated by Western Blotting in NIH3T3 cell nuclear extract.Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected ERK5 in NIH3T3 cell nuclear extract.Tested ApplicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected ERK5 in human spleen tissue sections.Affinity Binding Assay:: A representative lot of this antibody bound ERK5 peptide with a KD of 1.3 x 10-8 in an affinity binding assay.Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected recombinant Human ERK5.Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GST-tagged recombinant Human ERK5. Flow Cytometry Analysis: 0.1 µg from a representative lot detected ERK5 in one million A549 and HeLa cells.Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected ERK5 in Human spleen tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Mitogen-activated protein kinase 7 (UniProt: Q13164; also known as EC:2.7.11.24, MAP kinase 7, MAPK 7, Big MAP kinase 1, BMK-1, Extracellular signal-regulated kinase 5, ERK-5) is encoded by the MAPK7 (also known as BMK1, ERK5, PRKM7) gene (Gene ID: 5598) in human. ERK5 is an unusual protein kinase with a kinase domain (aa 55-347) and a transcriptional transactivation domain (aa 664-789). It is expressed in many adult tissues and higher expression is observed in heart, placenta, lung, kidney, and skeletal muscle. Although its kinase domain  displays high sequence identity with ERK1/2, they tend to have distinct substrates and unlike ERK1/2, it has a large C-terminal extension that contains a NuCLEAR™ localization signal (aa 505-539) and the transcriptional transactivation domain. ERK5 plays a role in various cellular processes, including cell proliferation, differentiation, and cell survival. Its upstream activator is MAP2K5 and following activation, it translocates to the nucleus and phosphorylates various downstream targets. It is activated by Dual® phosphorylation of threonine 219 and tyrosine 221. ERK5 is also important for endothelial function and maintenance of blood vessel integrity. ERK5-deficient mice die around embryonic day 10.5 because of cardiovascular defects and angiogenic failure in embryonic and extraembryonic tissues.  Four isoforms of ERK5 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity™, reproducibility, and stability over conventional monoclonals. (Ref.: (Ref.: Cook, SJ., and Lochhead, PA. (2022). Front. Cell Dev. Biol. 10; 839997; Yang, Q., et al. (2013). Oncogene. 32(26); 3156-3264; Nishimoto, S., and Nishida, E.  (2006). EMBO Rep. 7(8); 782-786).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: Affinity is a trademark of Mine Safety Appliances Co.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.