Anti-KIF5B Antibody, clone 2F23 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2F23 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Kinesin-1 heavy chain (KIF5B). It targets an epitope within 16 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Kinesin-1 heavy chain (KIF5B).
• Application: Quality Control TestingEvaluated by Western Blotting in C2C12 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected KIF5B in C2C12 cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected KIF5B in Human brain and Mouse brain tissue lysates.Affinity Binding Assay: A representative lot of this antibody bound KIF5B peptide with a KD of 4.6 x 10-6 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected KIF5B in HT-29 and U2OS cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected KIF5B in human kidney tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end userEvaluated by Western Blotting in C2C12 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected KIF5B in C2C12 cell lysate.
• Target description: Kinesin-1 heavy chain (UniProt: P33176; also known as Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, UKHC, KIF5B) is encoded by the KIF5B (also known as KNS, KNS1) gene (Gene ID: 3799) in human. Kinesin-1 is an abundant, ATP-dependent motor protein that moves along microtubule filaments. It participates in a number of important intracellular transport processes. Kinesin is a heterotetramer, comprised of 2 heavy chains and 2 light chains. The heavy chains dimerize to form an elongated stalk with 2 amino-terminal globular domains (motor domains) at one end and the light chains at the other end. Kinesin can pull a cellular cargo along a microtubule, slide one microtubule relative to another, or even remodel the microtubule cytoskeleton through regulation of microtubule dynamics. It plays a key role in mitosis and in the trafficking of organelles and vesicles. The Kinesin heavy chain is shown to move its cytoplasmic cargo toward the plus ends of microtubules. KIF5B is ubiquitous in its distribution and is shown to be uniformly distributed between soma and neurites in hippocampal neurons. It participates in the normal distribution of mitochondria and lysosomes in the cell and can induce formation of neurite-like membrane protrusions in non-neuronal cells in a Zinc Finger FYVE-Type Containing 27 (ZFYVE27)-dependent manner. It also regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle, it is reported to antagonize dynein function and drives the separation of nuclei and centrosomes. It is also required for anterograde axonal transportation of mitogen-activated protein kinase 8 interacting protein 3 (MAPK8IP3) and is essential for MAPK8IP3 function in axon elongation. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.