Anti-Catenin α-1 Antibody, clone 1D15 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1D15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Catenin -1. It targets an epitope within 17 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Catenin -1.
• Application: Quality Control TestingEvaluated by Western Blotting in Human Small Intestine tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Catenin -1 in Human Small Intestine tissue lysate.Tested ApplicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Catenin -1 in Human small intestine tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Catenin -1 peptide with a KD of 6.5 x 10-7 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Catenin alpha-1 (UniProt: P35221; also known as Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-Ren™-13) is encoded by the CTNNA1 gene (Gene ID: 1495) in human. Catenin α-1 is a ubiquitously expressed protein that can exist as a monomer of a homodimer. The monomeric form preferentially binds to catenin β1 and the homodimeric form binds to actin. Catenin α-1 associates with the cytoplasmic domain of a variety of cadherins, and this association produces a complex that is linked to the actin filament network and appears to be of importance for cadherins cell-adhesion properties. At cell junctions, it is essential for cell cohesion and tissue organization. It also plays a role in contact-dependent growth inhibition and can antagonize Wnt/β-catenin-mediated transcription. Usually, cells contain a significant amount of cadherin-free α-catenin, and this pool of free α-catenin can impact actin dynamics, such as limiting Arp2/3-based actin polymerization and severing by cofilin that ultimately promotes the assembly and stabilization of unbranched filament bundles. Loss of function of CTNNA1 gene has been reported in several cases of breast cancer and some cases of gastric cancers. Three isoforms of Catenin α-1 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity™, reproducibility, and stability over conventional monoclonals. (Ref.: Clark, DF., et al. (2020). Genet Med 22(5); 840-846; Daugherty, RL., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(14); 5260-5265).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: Affinity is a trademark of Mine Safety Appliances Co.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.