Anti-MXRA8 Antibody, clone 1F4 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1F4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects MXRA8. It targets an epitope within 18 amino acids from the extracellular domain within the C-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the extracellular domain within the C-terminal half of human Matrix remodeling-associated protein 8 (MXRA8).
• Application: Quality Control TestingEvaluated by Western Blotting in Mouse placenta tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MXRA8 in Mouse placenta tissue lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected MXRA8 in Human prostate tissue lysate.Affinity Binding Assay: A representative lot of this antibody bound MXRA8 peptide with a KD of 1.0 x 10-6 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MXRA8 in U2OS cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MXRA8 in Human prostate tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Matrix remodeling-associated protein 8 (UniProt: Q9BRK3; also known as Dual Ig domain-containing cell adhesion molecule, DICAM, MXRA8, Limitrin) is encoded by the MXRA8 gene (Gene ID: 54587) in human. MXRA8/DICAM is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 20-341), a transmembrane domain (aa 342-362), and a cytoplasmic domain (aa 363-442). It is highly expressed at the areas of cell-cell contacts. It is also detected in the soluble fraction of the nucleus. Its high expression is observed in the brain where it localizes to glia limitans formed by the end feet of astrocytes surrounding capillaries, and beneath the pia mater. It is also expressed in intercalated disks in the heart and has been detected in pancreatic -cells. It modulates the activity of various signaling pathways via binding to integrin V:β3 via i
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.