Anti-Claudin-5 Antibody, clone 3H12 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 3H12 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Claudin-5. It targets an epitope within 18 amino acids from the C-terminal, cytoplasmic domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal, cytoplasmic domain of human Claudin-5.
• Application: Quality Control TestingEvaluated by Western Blotting in MCF-7 cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Claudin-5 in MCF-7 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected Claudin-5 in Human colon tissue lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Claudin-5 in MCF-7 cells.Affinity Binding Assay: A representative lot of this antibody bound Claudin-5 peptide with a KD of 3.7 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Claudin-5 in Human kidney tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Claudin-5 (UniProt: O00501; also known as Transmembrane protein deleted in VCFS, TMDVCF) is encoded by the CLDN5 (also known as AWAL, TMVCF) gene (Gene ID: 7122) in human. Claudin-5 is a multi-pass membrane protein with three cytoplasmic domains (aa 1-7; 103-122; and 181-218); four transmembrane domains (aa 8-28, 82-102, 123-143, and 160-180); and two extracellular loops (aa 29-81 and 144-159). It plays a major role in tight junction-specific obliteration of the intercellular space. It is localized specifically to the endothelial cell layer in the brain, lung, liver, kidney, and skin. In peripheral tissues, it displays greater heterogeneity in tissue expression. At the blood-brain barrier, it is one of the most enriched tight junction proteins and its dysfunction has been linked to several neurodegenerative diseases, including Alzheimer s disease and multiple sclerosis. Cldn5-null mice are reported to die shortly after birth. This ZooMAb® recombinant monoclonal antibody, generated by
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.