Anti-Histamine Receptor 4 Antibody, clone 1E18 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1E18 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Histamine H4 receptor (HRH4). It targets an epitope within 18 amino acids from the C-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human Histamine H4 receptor (HRH4).
• Application: Quality Control TestingEvaluated by Western Blotting in K562 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Histamine Receptor 4 in K562 cell lysate.Tested Applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GST-tagged recombinant fragment corresponding to the third cytoplasmic domain of Human Histamine Receptor 4.Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected Histamine Receptor 4 in Human tonsil and Human dorsal root ganglion tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Histamine Receptor 4 in K562 cells.Affinity Binding Assay: A representative lot of this antibody bound Histamine Receptor 4 peptide with a KD of 1.6 x 10-8 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Histamine H4 receptor (UniProt: Q9H3N8; also known as H4R, HH4R, AXOR35, G-protein coupled receptor 105, GPRv53, Pfi-013, SP9144) is encoded by the HRH4 (also known as GPCR105) gene (Gene ID: 59340) in human. Histamine, an endogenous short-acting biogenic amine, is synthesized from histidine through the action of histidine decarboxylase. Its actions are mediated via four subtypes of G-protein-coupled receptors, designated as H1, H2, H3 and H4 histamine. Histamine H4 receptor (H4R) is a multi-pass membrane glycoprotein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. H4R mediates histamine signals in peripheral tissues and displays a significant level of constitutive activity. H4R is expressed on cells of the immune system and is also detected in a variety of peripheral tissues. Its expression is either up-regulated or down-regulated upon activation of the lymphoid tissues and this regulation may depend on the presence of IL-10 or IL-13. H4R m
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.