Anti-SUCNR1/GPR91 Antibody, clone 1N21 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1N21 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Succinate receptor 1 (SUCNR1/GPR91). It targets an epitope within 18 amino acids from the third cytoplasmic domain in the C-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the third cytoplasmic domain within the C-terminal half of human Succinate receptor 1 (SUCNR1/GPR91).
• Application: Quality Control TestingEvaluated by Immunohistochemistry (Paraffin) in Human breast cancer tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected SUCNR1/GRP91 in Human breast cancer tissue sections.Tested ApplicationsAffinity Binding Assay: A representative lot of this antibody bound SUCNR1/GPR91 peptide with a KD of 3.5 x 10^-8 in an affinity binding assay.Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SUCNR1/GPR91 in human adipose tissue lysates.Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected SUCNR1/GPR91 in SUCNR1 and GST Proteins.Immunofluorescence Analysis: A 1:5,000 dilution from a representative lot detected SUCNR1/GPR91 in Human liver and porcine kidney tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Succinate receptor 1 (UniProt: Q9BXA5; also known as G-protein coupled receptor 91, P2Y purinoceptor 1-like) is encoded by the SUCNR1 (also known as GPR91) gene (Gene ID: 56670) in human. Succinate receptor is a multi-pass membrane glycoprotein that belongs to the G-protein coupled receptor family with seven transmembrane domains. It serves as a receptor for succinate. SUCNR1 intracellular signaling is depended on the associated subunit type (G i, G s, and G q). Its expression is observed in kidney. Within the adaptive immune system, it has been detected in CD4+ and CD8+ T cells and in all subsets of B cells. T cells from patients with systemic lupus erythematosus (SLE) are reported to release large amounts of IL-10 and succinate following activation with oxidized mitochondrial DNA. Although its expression in the healthy CNS is reported to be low, its activation is considered as a key in the response of CNS cells to hypoxic-ischemic injury and inflammatory damage. In rodent retinal
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.