Anti-Ankyrin-G Antibody, clone 1D11 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1D11 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Ankyrin-G. It targets an epitope within 15 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Ankyrin-G.
• Application: Quality Control Testing Evaluated by Immunohistochemistry (Paraffin) in Human kidney tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Ankyrin-G in Human kidney tissue sections.Tested ApplicationsImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ankyrin-G in HEK293 cells.Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Ankyrin-G in T47D cell lysate.Affinity Binding Assay: A representative lot of this antibody bound Ankyrin-G peptide with a KD of 3.4 x 10-8 in an affinity binding assay.Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Ankyrin-G in Human kidney tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Ankyrin-3 (UniProt: Q12955; also known as ANK-3, Ankyrin-G) is encoded by the ANK3 gene (Gene ID: 288) in human. Ankyrins are a family of spectrin-binding scaffolding proteins that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins, which include channels and neuronal cell adhesion molecules. The ankyrin family of proteins consists of Ankyrin-R, Ankyrin-B, and Akyrin-G. Ankyrin-B and ankyrin-G have been detected in a variety of tissue. In contrast, ankyrin-R is detected only in erythrocytes, striated muscle, and some neurons. Ankyrin protein has four functional domains: a membrane-binding domain (MBD), spectrin-binding domain (SBD), death domain, and a C-terminal regulatory domain. Ankyrin-G is a involved in various protein interactions and neuronal functions, most notably the formation and maintenance of the axon initial segment and node of Ranvier of neurons. It links ion channels to the cytoskeleton in these areas and is critical for action potential generation and propagation. Its expression is observed in brain, muscles, and other tissues. Its levels are up-regulated during muscle cell differentiation. It may participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. In skeletal muscle it is required for costamere localization of dystrophin (DMD) and β-dystroglycan 1 (β-DAG1). It is reported to directly interact with DMD and β-DAG1, however, this interaction does not interfere with binding between DMD and β-DAG1. Mutations in ANK3 gene have been linked to bipolar disorder and other psychiatric disorders. Five isoforms of Ankyrin-G have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Salzer, JL. (2019). Proc. Natl. Acad. Sci. USA. 116(39); 19228-19230; Luoni, A., (2016). Transl. Psychiatry.e943; Iqbal, Z., et al. (2013). Hum. Mol. Genet. 22(10); 1960-1970).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.