Anti-ATP Synthase β Antibody, clone 1E6 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1E6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects ATP Synthase β. It targets an epitope within 17 amino acids from the internal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 17 amino acids from the internal region of human ATP Synthase β.
• Application: Quality Control TestingEvaluated by Western Blotting in Rat heart tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ATP Synthase β in Rat heart tissue lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected ATP Synthase β in lysates from A431 and NIH3T3, cells and Human liver tissue lysates.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ATP Synthase β in Human liver tissue sections.Flow Cytometry Analysis: 0.1 µg from a representative lot detected ATP Synthase β in one million HL60 cells.Affinity Binding Assay: A representative lot of this antibody bound ATP Synthase β peptide with a KD of 8.8 x 10-8 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ATP Synthase β in A431 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: ATP synthase subunit beta, mitochondrial (UniProt: P06576; also known as EC:7.1.2.2, ATP synthase F1 subunit beta) is encoded by the ATP5F1B (also known as ATP5B, ATPMB, ATPSB) gene (Gene ID: 506) in human. ATP Synthase β is a mitochondrial inner membrane protein that is synthesized with a transit peptide (aa 1-47), which is subsequently cleaved off. It is part of the F-type ATPase that has two structural domains: F1 domain that contains the extra-membraneous catalytic core; and the F0 domain that contains the membrane proton channel. These two domains are linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits and β form the catalytic core in F1. ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane that is generated by electron transport complexes of the respiratory chain. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Galber, C., et al. (2021). Cell Rep. 35(6); 109111).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.