Anti-SLC31A1/CTR1 Antibody, clone 1K21 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1K21 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects High affinity copper uptake protein 1 (SLC31A1). It targets an epitope within 18 amino acids from the N-terminal extracellular domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal, extracellular domain of human High affinity copper uptake protein 1 (SLC31A1).
• Application: Quality Control TestingEvaluated by Western Blotting in Human liver cell membrane preparations.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC31A1/CTR1 in Human liver cell membrane preparations.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SLC31A1/CTR1 in Jurkat cell membrane preparations.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SLC31A1/CTR1 in HepG2 cells.Affinity Binding Assay: A representative lot of this antibody bound SLC31A1/CTR1 peptide with a KD of 1.0 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: High affinity copper uptake protein 1 (UniProt: O15431; also known as Copper transporter 1, hCTR1, Solute carrier family 31 member 1) is encoded by the SLC31A1 (also known as COPT1, CTR1) gene (Gene ID: 1317) in human. SLC31A1 is a homotrimeric, multi-pass membrane protein with two extracellular domains (aa 1-61; 154-156), three transmembrane domains (aa 62-82; 133-153; 157-177), and two cytoplasmic domains (aa 83-132; 178-190). It serves as a high-affinity copper uptake protein that transports copper1+ from extracellular space to the cytoplasm and delivers to specific chaperones, such as Antioxidant 1 Copper Chaperone (ATOX1). The copper1+ transport mechanism is reported to be sodium-independent and is saturable. This transport is stimulated by extracellular acidic pH and high levels of potassium ions. Copper1+ import is reported to be regulated by a copper1+-dependent recycling of SLC31A1. It also participates in the first step of copper2+ acquisition by cells through a direct transfer of copper2+ from copper2+ carriers in blood then leading to copper2+ reduction and stabilization of copper1+. Its C-terminal domain is known to mediate copper1+ binding and is involved in the copper1+-dependent-ATOX1 interaction. It also limits transport through the pore by regulating the rate of exit of copper ions. Its N-terminal domain can collect copper2+ from copper2+ carriers in blood. SLC21A1 interacts with SLC31A2, and this interaction is shown to stabilize SLC31A2 and protect it from ubiquitination and degradation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Stefaniak, E., et al. (2018). Metallomics. 10(12); 1723-1727; Maryon, EB., et al. (2013). J. Biol. Chem. 288(25); 18035-18046; Lee, J., et al. (2002). J. Biol. Chem. 277(6); 4380-4387).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.