EZHIL8

Human IL-8 ELISA Kit

detect IL-8 in 50 μL serum, plasma or cell culture supernatent

Manufacturer: Sigma Aldrich

Synonym(S): Granulocyte chemotactic protein 1, IL-8, Interleukin-8, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, T-cell chemotactic factor

Select a Size

Pack Size SKU Availability Price
1 PKG EZHIL8-1-PKG In Stock ₹ 59,010.00
5 X 1 PKG EZHIL8-5-X-1-PKG In Stock ₹ 3,24,723.84
10 X 1 PKG EZHIL8-10-X-1-PKG In Stock ₹ 5,89,774.08

EZHIL8 - 1 PKG

₹ 59,010.00

In Stock

Quantity

1

Base Price: ₹ 59,010.00

GST (18%): ₹ 10,621.80

Total Price: ₹ 69,631.80

product name

Human IL-8 Conferma® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples

Quality Level

200

species reactivity

human

parameter

50 μL sample volume (4hr assay)

assay range

accuracy: 77-102 (plasma)%linearity: 105 (plasma)%inter-assay cv: <4.7%intra-assay cv: <4.0%standard curve range: 0.82-200 pg/mLaccuracy: 70-105 (serum)%linearity: 94-106 (serum)%

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)sample type cell culture supernatantsample type serum

UniProt accession no.

P10145

application(s)

research use

detection method

colorimetric (450nm/590nm)

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Description

  • General description: IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.This is a 96 well format sandwich ELISA which uses our “Conferma” process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.
  • Application: This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples.Analytes available:IL-8Research Category: CytokinesInflammation & ImmunologyImmune ResponseMetabolic Disease
  • Analysis Note: 3 hour 45 minute assay, room temperature, 50μl of neat serum or plasma is recommened.
  • Legal Information: CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany
  • Disclaimer: For research use only. Not for use in diagnostic procedures.

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Sigma Aldrich

EZHIL8

detect IL-8 in 50 μL serum, plasma ...


product name:
Human IL-8 Conferma® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples

Quality Level:
200

species reactivity:
human

parameter:
50 μL sample volume (4hr assay)

assay range:
accuracy: 77-102 (plasma)%linearity: 105 (plasma)%inter-assay cv: <4.7%intra-assay cv: <4.0%standard curve range: 0.82-200 pg/mLaccuracy: 70-105 (serum)%linearity: 94-106 (serum)%

technique(s):
ELISA: suitable

input:
sample type plasma (K2 EDTA)sample type cell culture supernatantsample type serum

UniProt accession no.:
P10145

application(s):
research use

detection method:
colorimetric (450nm/590nm)

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200

species reactivity:
human

parameter:
25 μL sample volume (4hr assay)

assay range:
accuracy: 94-114%intra-assay cv: 1.4-4.9(Sensitive Assay)intra-assay cv: 2.6-4.6linearity: 80-105%linearity: 96-125%(sensitive assay)sensitivity: 0.125 ng/mL(sensitive assay)sensitivity: 0.125-20 ng/mLsensitivity: 0.5 ng/mLstandard curve range: 0.5-100 ng/mL

technique(s):
ELISA: suitable

input:
sample type cell culture supernatantsample type plasma (K2 EDTA)sample type serum

UniProt accession no.:
P41159

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technique(s):
ELISA: suitable

input:
sample type plasma (K2 EDTA)sample type serum

UniProt accession no.:
P01303

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parameter:
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assay range:
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technique(s):
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input:
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UniProt accession no.:
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