Protein G Sepharose™, Fast Flow

Description

• General description: Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148.[1] This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200.[2] The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10.[3] It acts as a powerful reagent for the detection of IgG.[1]
• Application: Protein G-Sepharose™ is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose™ has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum.
• Physical form: Suspension in 20% ethanol
• Preparation Note: Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted
• Legal Information: Sepharose is a trademark of Cytiva