S1638

Streptavidin−Agarose from Streptomyces avidinii

buffered aqueous suspension

Manufacturer: Millipore

Synonym(S): streptavidin agarose beads, streptavidin agarose resin

Select a Size

Pack Size SKU Availability Price
1 ML S1638-1-ML In Stock ₹ 19,090.00
5 ML S1638-5-ML In Stock ₹ 70,827.98

S1638 - 1 ML

₹ 19,090.00

In Stock

Quantity

1

Base Price: ₹ 19,090.00

GST (18%): ₹ 3,436.20

Total Price: ₹ 22,526.20

form

buffered aqueous suspension

Quality Level

200

extent of labeling

≥1 mg per mL

technique(s)

affinity chromatography: suitable

matrix

4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

7 atoms

capacity

≥15 μg/mL binding capacity (biotin)

storage temp.

2-8°C

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Description

  • Application: Streptavidin−agarose from Streptomyces avidinii has been used:to pull down biotinylated cell surface proteins during the quantification of plasma membrane transforming growth factor β (TGFβ) receptor II (TβRII) and TβRII internalizationin biotinylated miRNA pull-down assay; as secondary antibodies in immunoprecipitation
  • Biochem/physiol Actions: Streptavidin is a homotetrameric protein, isolated from Streptomyces avidinii, which, like avidin, has a high affinity for biotin. Streptavidin is slightly anionic (pI ~ 5-6) and non-glycosylated. These properties contribute to its relatively low non-specific binding compared to egg white avidin. Streptavidin is also more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin-agarose can be used to immobilize or isolate various biotinylated macromolecules and complexes (proteins, antibodies, lectins, nucleic acids, receptors, and ligands). The inherent high-affinity streptavidin-biotin interaction requires harsh conditions to release biotinylated macromolecules. This feature makes streptavidin-agarose useful in a variety of affinity purification applications.
  • Physical form: Suspension in 0.01 M sodium phosphate, pH 7.2, containing 0.05 M NaCl and 0.02% sodium azide

SAFETY INFORMATION

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Show Difference

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Millipore

S1638

buffered aqueous suspension...


form:
buffered aqueous suspension

Quality Level:
200

extent of labeling:
≥1 mg per mL

technique(s):
affinity chromatography: suitable

matrix:
4% beaded agarose

matrix activation:
cyanogen bromide

matrix attachment:
amino

matrix spacer:
7 atoms

capacity:
≥15 μg/mL binding capacity (biotin)

storage temp.:
2-8°C

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Sigma Aldrich

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lyophilized powder...


form:
lyophilized powder

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200

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__

matrix activation:
__

matrix attachment:
__

matrix spacer:
__

capacity:
__

storage temp.:
−20°C

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Sigma Aldrich

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matrix activation:
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matrix attachment:
__

matrix spacer:
__

capacity:
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storage temp.:
room temp

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Sigma Aldrich

S1647

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200

extent of labeling:
__

technique(s):
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__

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__

capacity:
__

storage temp.:
room temp