P7457

Carboxy-terminal FLAG-BAP™ Fusion Protein

Manufacturer: Sigma Aldrich

Select a Size

Pack Size SKU Availability Price
0.1 MG P7457-0.1-MG In Stock ₹ 41,211.60

P7457 - 0.1 MG

₹ 41,211.60

In Stock

Quantity

1

Base Price: ₹ 41,211.60

GST (18%): ₹ 7,418.088

Total Price: ₹ 48,629.688

form

liquid

Quality Level

200

mol wt

~49 kDa

shipped in

dry ice

storage temp.

−20°C

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Description

  • General description: Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).
  • Application: Carboxy-terminal FLAG-BAP™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate[1] and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).[2]
  • Biochem/physiol Actions: The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification.[3] Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.[4]
  • Other Notes: Control protein
  • Physical form: Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2
  • Preparation Note: Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml
  • Legal Information: FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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