Peroxidase (POD)

Description

• General description: Donor: hydrogen-peroxide oxidoreductasePeroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.
• Application: Peroxidase (POD) has been used in the spermine oxidase enzyme activity assay.[1]
• Sequence: Blocking substancesPreparations does not contain substances that block the amino groups, but in contrast to other enzymes POD has only 2 to 3 free amino groups which are not accessible; even by extended dialysis of POD, the number of NH2-groups/mol does not change.PODPOD contains 1 molecule of protoheme IX as prosthetic group and 2 molecules of Ca2+.POD exists in > 10 isoenzymic forms (some sources estimate approx. 40 isoenzymes).Carbohydrate Content12 to 14.5 mg/100 mg IyophilizateFree Amino GroupsFree amino groups: 2 to 3 mol/mol enzyme
• Preparation Note: Activator: Ammonia, pyridine, imidazole, all at pH >7; detergentsWorking concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).Specific activity: approximately 225U/mg lyophilizate (+25°C, guaiacol, H2O2) approximately 1,000 U/mg lyophilizate (+25°C, ABTS, H2O2, pH 5.0) approximately 3,500 U/mg lyophilizate (+25°C, tetramethylbenzidine, H2O2)Amino groups: 2 - 3moles/mole enzyme
• Storage and Stability: Keep container tightly closed in a dry and well-ventilated place.
• Other Notes: For life science research only. Not for use in diagnostic procedures.