Quantification kit for oxidized and reduced glutathione

Description

• General description: Glutathione Quantification Kit is designed to detect oxidized and reduced Glutathione. Glutathione is usually represented in a reduced form called GSH; however, GSH is converted into its oxidized state (GSSG) upon oxidative stress. The ratio between GSH and GSSG is considered an index for oxidative stress. Glutathione, also known as γ-L-glutamyl-L-cysteinylglycine, is a tripeptide compound that is involved in anti-oxidation and drug metabolism. It acts as a substrate for enzymes like glutathione peroxidase, glutathione S-transferase, and thiol transferase.
• Application: Glutathione Quantification Kit is used to detect the total amount of glutathione as an index for oxidative stress or disease risk.
• Features and Benefits: The detection ranges of total glutathione and GSSG using in this kit are from 0.5 μmol/l to 50 μmol/l and from 0.5 μmol/l to 25 μmol/l, respectively.
• Components: Glutathione Quantification Kit contains: Enzyme Solution (50µl X 1) Buffer Solution (60ml X 1) Standard GSH (X 1) Masking Reagent (20µl X 1) Co-enzyme (X 2) Substrate (DTNB) (X4) Standard GSSG (X 1)
• Analysis Note: Glutathione Quantification Kit contains a GSH masking reagent. This enables GSSG detection in the sample by measuring the absorption (λmax = 412 nm) obtained from a colorimetric reaction of DTNB (5,5’-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system. The total quantity of GSH can be measured by subtracting the amount of GSSG from the total amount of glutathione.
• Other Notes: not available in Korea, Singapore, Taiwan and Japan