GlycoProfile™ IV Chemical Deglycosylation Kit

Description

• General description: The GlycoProfile™ IV Kit utilizes trifluoromethane sulfonic acid (TFMS) in a deglycosylation system that completely removes all N- and O-linked glycans while safeguarding the protein or polypeptide structure. TFMS hydrolysis of glycoproteins results in minimal protein degradation while the released glycans are eliminated. The conditions are optimized to deglycosylate simple or low molecular weight glycoproteins in 30 mins without the scavenger. For high molecular weight or complex, non-mammalian glycoproteins, anhydrous anisole is provided as a scavenger to give a high protein yield.
• Application: GlycoProfile™ IV Chemical Deglycosylation Kit has been used in the deglycosylation of human α-L-iduronidase[1] and peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera.[2] It has also been used to estimate the glycosylation of plant-derived recombinant human interleukin-13 (rhIL-13).[3]
• Features and Benefits: Eliminates both N- and O-linked glycans from proteinsThis enables sequence analysis of the protein core by mass spectrometry (MS) or DNA-to-protein sequence comparisonEliminates glycans which affect the molecular radiusAllows molecular mass determination by electrophoresisComplete deglycosylation in as short as 30 mins for high throughputQuick as it eliminates the need for multiple enzyme reactionsAccommodates a broad range of glycoproteinsMinimal degradation of the protein core to facilitate reliable MS results
• Quantity: The kit contains sufficient reagents for 10 reactions with a sample size between 1-2 mg of a typical glycoprotein. An RNase B glycoprotein standard is included.
• Legal Information: GlycoProfile is a trademark of Sigma-Aldrich Co. LLC