GlycoProfile™ Glycan Clean-Up Cartridge

Description

• General description: Glycan analysis has become an increasingly critical aspect of glycomics and proteomics, due to the role of glycoproteins in cell signaling, cell adhesion, immune response, and disease states.[1] Since they tend to have low spectral activity in both UV and visible light, it is often necessary to label glycans in order to enhance detection. GlycoProfile™ Glycan Clean-Up Cartridges are designed for the post-labeling cleanup of labelled glycans when using the GlycoProfile™ 2-AA or 2-AB Labeling Kits. Glycans adsorb to the membrane while excess dye and other hydrophobic contaminants pass through. Mono- and disaccharides have a weak affinity for the cartridge membrane and tend to bind poorly. Purified glycans are subsequently desorbed and ready for further enzymatic digestion or analysis by a number of methods, including HPLC.
• Application: GlycoProfile™ Glycan Clean-Up Cartridge is used for clean up of glycan samples after reductive amination labeling[2] or enzymatic digestion. It has also been used to remove excess dye and hydrophobic contaminants from N-linked glycans.[3] The cartridge is equilibrated with acetonitrile, then loaded with a maximum of 20 μg of a glycan sample. The glycans are bound while excess dye and salts are removed by washing with acetonitrile. The glycans are then eluted with water.
• Linkage: Recommended for use with the GlycoProfile 2-AA (Cat. No. PP0530) and 2-AB (Cat. No. PP0520) Labeling Kits.
• Storage and Stability: Store the cartridges at room temperature. The cartridges are stable for at least one year at room temperature, as supplied.
• Other Notes: Each cartridge is recommended for single use only. The maximum sample size is 10 μL of glycan containing solution and/or 20 μg of glycan.
• Legal Information: GlycoProfile is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
• Disclaimer: This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.