GlycoProfile™ 2-AB Labeling Kit

Description

• General description: The GlycoProfile™ labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosyl­phosphatidyl­inositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.
• Application: GlycoProfile™ 2-AB Labeling Kit has been used for the labelling of N-linked glycans from human IgG, Bovine fetuin, and[1] human epididymis protein 4.[2] It has also been used for labelling free oligosaccharides from mouse spleen.[3]
• Legal Information: Labeling of glycans with 2-AB is covered under US Patent No. 5,747,347 and its foreign equivalents.