N-Glycosidase F

Description

• General description: Peptide-N-glycosidase F, Peptide-N4-(acetyl-β-glucosaminyl)-asparagine amidase
• Application: Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit.[1][2][3] The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.
• Unit Definition: One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8.
• Physical form: Solution in 50 mM sodium phosphate, 12.5 mM EDTA, 50% glycerol (v/v), pH 7.2
• Other Notes: For life science research only. Not for use in diagnostic procedures.
• Legal Information: The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.