N-Glycosidase F

Description

• General description: N-Glycosidase F (PNGase F) is a potent enzyme which hydrolyzes at glycosylamine linkage. It also helps in generating a carbohydrate-free peptide and oligosaccharide with di-N-acetylchitobiose unit.[1]N-glycosidase F, also known as PNGase F, is an asparagine amidase enzyme derived from Flavobacterium meningosepticum. It is widely used as a valuable tool in protein research to investigate and analyze N-glycosylation.[2]
• Specificity: Hydrolyzes all types of N-glycan chains from glycopeptides and glycoproteins unless they carry α1,3-linked core fucose residues present in insect and plant glycoproteins. Free of contaminating proteolytic activities (x = H or sugar[s]) according to current quality control procedures.
• Application: N-Glycosidase F has been used for deglycosylation of N-glycoproteins.[3][4]Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.Note: N-Glycosidase F, recombinant is also available as a solution.
• Unit Definition: One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycoprotein within 1 minute at 37 °C and pH 7.8.
• Physical form: Clear, colorless solution after reconstitution
• Preparation Note: Storage conditions (working solution): 2 to 8 °CThe reconstituted solution is stable at 2 to 8 °C for at least four weeks.
• Reconstitution: Dissolving the content in 0.1 ml redist water (100 unit package) or 0.25 ml double-dist. water (250 unit package) respectively, results in a concentration of 100 mM sodium phosphate buffer, 25 mM EDTA, pH 7.2.Note: N-Glycosidase F, recombinant is also available as solution with 50% glycerol.
• Other Notes: For life science research only. Not for use in diagnostic procedures.