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P4069

Phosphatase, Alkaline from Escherichia coli

Name: Phosphatase, Alkaline from Escherichia coli | Sigma Aldrich | Chemikart
Brand: Chemikart

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

Manufacturer: Sigma Aldrich

CAS Number: 9001-78-9

Synonym(S): Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

Select a Size

Pack Size	SKU	Availability	Price
25 UNITS	P4069-25-UNITS	In Stock	₹ 36,829.80
100 UNITS	P4069-100-UNITS	In Stock	₹ 68,276.10

P4069 - 25 UNITS

₹ 36,829.80

In Stock

Quantity

Base Price: ₹ 36,829.80

GST (18%): ₹ 6,629.364

Total Price: ₹ 43,459.164

biological source

Escherichia coli

Quality Level

200

form

buffered aqueous glycerol solution

specific activity

20-50 units/mg protein (in glycine buffer)

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Other Options

Product Name	Manufacturer	Price Range
Alkaline Phosphatase, MP Biomedicals™	MP Biomedicals, Inc	₹ 69,132.48
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa, 9001-78-9, MFCD00131849, 500 mg	Sigma Aldrich Fine Chemicals Biosciences	₹ 13,521.05
Sigma Aldrich Fine Chemicals Biosciences Alkaline Phosphatase Recombinant solution (high-activity) \| 9001-78-9 \| 15MG	Sigma Aldrich Fine Chemicals Biosciences	₹ 1,46,393.16
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein \| 9001-78-9 \| MFCD00131849 \| 2KU	Sigma Aldrich Fine Chemicals Biosciences	₹ 14,254.30
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, >=4,000 DEA units/mg protein \| 9001-78-9 \| MFCD00131849 \| 2KU	Sigma Aldrich Fine Chemicals Biosciences	₹ 19,285.22
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa BioUltra, buffered aqueous glycerol solution, ≥6,000 DEA units/mg protein \| 9001-78-9 \| MFCD00131849 \| 10ku	Sigma Aldrich Fine Chemicals Biosciences	₹ 58,933.73
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa >=2,000 DEA units/mg protein \| 9001-78-9 \| MFCD00131849 \| 2KU	Sigma Aldrich Fine Chemicals Biosciences	₹ 17,084.62
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa >=2,000 DEA units/mg protein \| 9001-78-9 \| MFCD00131849 \| 10KU	Sigma Aldrich Fine Chemicals Biosciences	₹ 56,572.27
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein \| 9001-78-9 \| MFCD00131849 \| 1KU	Sigma Aldrich Fine Chemicals Biosciences	₹ 9,590.42
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous glycerol solution, ≥5,500 DEA units/mg protein \| 9001-78-9 \| MFCD00131849 \| 50ku	Sigma Aldrich Fine Chemicals Biosciences	₹ 95,508.92

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Description

Application: Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.
Biochem/physiol Actions: Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).[1]
Caution: Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.
Unit Definition: One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.
Physical form: Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl2, 0.5 mM ZnCl2, 50% (w/v) glycerol
Analysis Note: Protein determined by biuret.

SAFETY INFORMATION

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sigma Aldrich

P4069

buffered aqueous glycerol solution,...

biological source:

Escherichia coli

Quality Level:

200

form:

buffered aqueous glycerol solution

specific activity:

20-50 units/mg protein (in glycine buffer)

foreign activity:

DNase, RNase, none detected

storage temp.:

−20°C

Sigma Aldrich

P4083

liquid, suitable for cell culture, ...

biological source:

Quality Level:

300

form:

liquid

specific activity:

foreign activity:

__

storage temp.:

−20°C

Sigma Aldrich

P4083

liquid, suitable for cell culture, ...

biological source:

Quality Level:

300

form:

liquid

specific activity:

foreign activity:

storage temp.:

Sigma Aldrich

P4099900

European Pharmacopoeia (EP) Referen...

Phosphatase, Alkaline from Escherichia coli

Select a Size

Select a Size

Properties

biological source

Quality Level

form

specific activity

foreign activity

storage temp.

Other Options

Related Products

Sigma Aldrich

Sigma Aldrich

Sigma Aldrich

Sigma Aldrich

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Sigma Aldrich

Sigma Aldrich

Sigma Aldrich

Description

SAFETY INFORMATION

Pictograms

Signal Word

Hazard Statements

Precautionary Statements

Hazard Classifications

WGK

Flash Point(F)

Flash Point(C)

Personal Protective Equipment

Compare Similar Items

Show Difference

Sigma Aldrich

biological source: Escherichia coli Quality Level: 200 form: buffered aqueous glycerol solution specific activity: 20-50 units/mg protein (in glycine buffer) foreign activity: DNase, RNase, none detected storage temp.: −20°C

Sigma Aldrich

biological source: __ Quality Level: 300 form: liquid specific activity: __ foreign activity: __ storage temp.: −20°C

Sigma Aldrich

biological source: __ Quality Level: 300 form: liquid specific activity: __ foreign activity: __ storage temp.: __

Sigma Aldrich

biological source: __ Quality Level: __ form: __ specific activity: __ foreign activity: __ storage temp.: 2-8°C

biological source:

Escherichia coli

Quality Level:

200

form:

buffered aqueous glycerol solution

specific activity:

20-50 units/mg protein (in glycine buffer)

foreign activity:

DNase, RNase, none detected

storage temp.:

−20°C

biological source:

Quality Level:

300

form:

liquid

specific activity:

foreign activity:

__

storage temp.:

−20°C

biological source:

Quality Level:

300

form:

liquid

specific activity:

foreign activity:

storage temp.:

biological source:

Quality Level:

form:

specific activity:

foreign activity:

__

storage temp.:

2-8°C