Description

• General description: Trehalase is a glycosidase[1] that occurs in many forms and is found in all tissues.[2] It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.[1][3]
• Application: Trehalase has been used:in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves[5]in a study to investigate growth arrest by trehalose-6-phosphate[6]to quantify the intracellular trehalose in Lactobacillus casei cells[7]
• Biochem/physiol Actions: Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.[2]
• Unit Definition: One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).
• Physical form: Solution in 50% glycerol containing 1% Triton™ X-100 and 25 mM potassium phosphate, pH 6.5
• Legal Information: Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow