P7923
Phosphatase, Alkaline from bovine intestinal mucosa
buffered aqueous glycerol solution, ≥4,000 DEA units/mg protein
Manufacturer: Sigma Aldrich
CAS Number: 9001-78-9
Synonym(S): Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 2000 UNITS | P7923-2000-UNITS | In Stock | ₹ 18,259.50 |
| 10000 UNITS | P7923-10000-UNITS | In Stock | ₹ 61,782.60 |
P7923 - 2000 UNITS
In Stock
Quantity
1
Base Price: ₹ 18,259.50
GST (18%): ₹ 3,286.71
Total Price: ₹ 21,546.21
form
buffered aqueous glycerol solution
Quality Level
200
specific activity
≥4,000 DEA units/mg protein
mol wt
dimer ~160 kDa
concentration
≥10.0 mg/mL
storage temp.
2-8°C
Other Options
Related Products
Description
- Application: Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
- Biochem/physiol Actions: Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It requires zinc, and magnesium or calcium divalent ions for activity[4].
- Physical properties: Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
- Unit Definition: One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)
- Physical form: Solution in 40% glycerol containing 6 mM Tris/HCl, 6 mM MgCl2 and 0.12 mM ZnCl2, ~pH 7.6
- Analysis Note: Package sizes are based on DEA units
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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form: buffered aqueous glycerol solution
Quality Level: 200
specific activity: ≥4,000 DEA units/mg protein
mol wt: dimer ~160 kDa
concentration: ≥10.0 mg/mL
storage temp.: 2-8°C
form:
buffered aqueous glycerol solution
Quality Level:
200
specific activity:
≥4,000 DEA units/mg protein
mol wt:
dimer ~160 kDa
concentration:
≥10.0 mg/mL
storage temp.:
2-8°C
form: lyophilized powder
Quality Level: 200
specific activity: __
mol wt: __
concentration: __
storage temp.: −20°C
form:
lyophilized powder
Quality Level:
200
specific activity:
__
mol wt:
__
concentration:
__
storage temp.:
−20°C
form: viscous liquid
Quality Level: 300
specific activity: __
mol wt: ~1228
concentration: __
storage temp.: __
form:
viscous liquid
Quality Level:
300
specific activity:
__
mol wt:
~1228
concentration:
__
storage temp.:
__
form: __
Quality Level: __
specific activity: __
mol wt: __
concentration: __
storage temp.: __
form:
__
Quality Level:
__
specific activity:
__
mol wt:
__
concentration:
__
storage temp.:
__