Filipin complex ready made solution

Description

• General description: Filipin Complex, the collective name given to family of isomeric polyene macrolides isolated from cultures of S. filipinensis, with Filipin III being the majority part of the complex.[1]
• Application: Filipin complex readymade solution has been used in the staining of HeLa cells for cytoskeletal imaging.[2]
• Biochem/physiol Actions: Filipin is an antibiotic with antifungal functionality.[3] Filipin complex has been found to alter cell membrane structure by interacting with the membrane sterols ergosterols and cholesterol.[1] On binding to cholesterol, filipin shows fluorescence shift. As such, filipin complex has become a useful tool for diagnosis of Niemann-Pick type C disease, and for detection and quantification of cholesterol in cell membranes and lipid quantification in brain.[3]
• Other Notes: Filipin complex ready made solution is very sensitive to air and light. Upon receipt aliquot and store at -20°C. Avoid freeze thaw cycles.Filipin complex is used in various concentrations depending on the specific protocol used. Dilute 5mg/ml stock solution with appropriate buffer according to the used protocol.Filipin complex interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).