3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

Description

• Application: TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
• Caution: This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.
• Physical form: Ready-to-use.
• Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research