V900498
Ribonuclease A from bovine pancreas
Vetec™, reagent grade, 80%
Manufacturer: Sigma Aldrich
CAS Number: 9001-99-4
Synonym(S): Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 MG | V900498-25-MG | In Stock | ₹ 5,883.00 |
| 100 MG | V900498-100-MG | In Stock | ₹ 18,037.50 |
V900498 - 25 MG
In Stock
Quantity
1
Base Price: ₹ 5,883.00
GST (18%): ₹ 1,058.94
Total Price: ₹ 6,941.94
biological source
bovine pancreas
grade
reagent grade
product line
Vetec™
Assay
80%
form
powder
specific activity
≥50 Kunitz units/mg protein
mol wt
~13,700
technique(s)
cell based assay: suitable
storage temp.
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
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Description
- General description: RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.
- Application: RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.RNase A is also used in RNA sequence analysis and protection assays.RNase A has been used as a tool for computer-aided drug design.RNase A supports the analysis of RNA sequences.RNase A hydrolyze RNA contained in protein samples.Purification of DNA is supported by RNase A.
- Features and Benefits: Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.
- Analysis Note: Protein determined by E.
- Legal Information: Vetec is a trademark of Merck KGaA, Darmstadt, Germany
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Compare Similar Items
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biological source: bovine pancreas
grade: reagent grade
product line: Vetec™
Assay: 80%
form: powder
specific activity: ≥50 Kunitz units/mg protein
mol wt: ~13,700
technique(s): cell based assay: suitable
storage temp.: −20°C
InChI: 1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
biological source:
bovine pancreas
grade:
reagent grade
product line:
Vetec™
Assay:
80%
form:
powder
specific activity:
≥50 Kunitz units/mg protein
mol wt:
~13,700
technique(s):
cell based assay: suitable
storage temp.:
−20°C
InChI:
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
biological source: __
grade: reagent grade
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Assay: __
form: powder
specific activity: ≥250 units/mg solid
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technique(s): __
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biological source:
__
grade:
reagent grade
product line:
Vetec™
Assay:
__
form:
powder
specific activity:
≥250 units/mg solid
mol wt:
~44 kDa
technique(s):
__
storage temp.:
2-8°C
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1S/H2O3/c1-3-2/h1-2H
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