18-033-100
Invitrogen™ T7 RNA Polymerase
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-033-100-Each-of-1 | In Stock | ₹ 56,070.00 |
18-033-100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 56,070.00
GST (18%): ₹ 10,092.60
Total Price: ₹ 66,162.60
Content And Storage
T7 RNA Polymerase (50 U/μL) is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl2, 10 mM spermidine-(HCl)3, 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.
Polymerase
T7 RNA Polymerase
Promoter
T7
Quantity
2 x 2500 U
Related Products
Description
- Synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter
- A T7 RNA Polymerase technical bulletin is available
- Application: Synthesis of labeled and unlabeled RNA transcripts
- Source: Purified from E
- coli expressing the T7 RNA Polymerase gene on a plasmid Performance and Quality Testing: 3' and 5' exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction Unit Definition: One unit hydrolyzes 1nMol of ribonucleotide into acid-precipitable material in 1 hr at 37°C using a T7 transcription vector as template Unit Reaction Conditions: 40mM Tris-HCl (pH 8.0), 25mM NaCl, 8mM MgCl 2 , 2mM spermidine-(HCl) 3 , 5mM DTT, 0.4mM ATP, 0.4mM CTP, 0.4mM GTP, 0.4mM UTP, 1μCi [ 3H]GTP, 1μg Sph I-cut pT7L13, and enzyme in 50μL for 10 min
- at 37°C Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Wet Ice
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Content And Storage: T7 RNA Polymerase (50 U/μL) is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl2, 10 mM spermidine-(HCl)3, 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.
Polymerase: T7 RNA Polymerase
Promoter: T7
Quantity: 2 x 2500 U
Content And Storage:
T7 RNA Polymerase (50 U/μL) is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl2, 10 mM spermidine-(HCl)3, 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.
Polymerase:
T7 RNA Polymerase
Promoter:
T7
Quantity:
2 x 2500 U
Content And Storage: __
Polymerase: __
Promoter: __
Quantity: __
Content And Storage:
__
Polymerase:
__
Promoter:
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Quantity:
__
Content And Storage: __
Polymerase: __
Promoter: __
Quantity: 1 Ea.
Content And Storage:
__
Polymerase:
__
Promoter:
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Quantity:
1 Ea.
Content And Storage: •TaqDNA Polymerase (5 U/μL), 20 μL• 10X PCR buffer (without magnesium), 1.25 mL• 50 mM MgCl2, 1 mLStore at -20°C in a non-frost-free freezer.Stable for 6 months when stored properly.
Polymerase: TaqDNA Polymerase
Promoter: __
Quantity: 100 Units
Content And Storage:
•TaqDNA Polymerase (5 U/μL), 20 μL• 10X PCR buffer (without magnesium), 1.25 mL• 50 mM MgCl2, 1 mLStore at -20°C in a non-frost-free freezer.Stable for 6 months when stored properly.
Polymerase:
TaqDNA Polymerase
Promoter:
__
Quantity:
100 Units