Thermo Scientific™ Poly(A) Polymerase, Yeast

Description

• The reaction requires Mn 2+ or Mg 2+ , ATP as substrate, and any RNA containing 3' hydroxyl termini as primer
• Substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single 3'-dA residue to the ends of the RNA, a useful technique for labeling RNA at the 3' end
• In comparative studies, yeast poly(A) polymerase works more efficiently than E
• coli poly(A) polymerase for RNA oligonucleotide-labeling and poly(A) tailing
• Poly(A) Polymerase is recommended over T4 RNA Ligase for 3'-end labeling of long RNA molecules
• Ribonuclease free 20 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT, 50% glycerol
• One unit equals 15 pmol/min AMP incorporated into (riboA)15 at 37°C
• 600 units/μL 3' -end labeling of a ribonucleotide with cordycepin-5'-triphosphate
• Addition of poly(A) tails to RNA.;2.Labeling the 3' ends of RNA
• E
• coli strain containing an overproducing clone of Yeast Poly(A) Polymerase.<br