Thermo Scientific™ T7 RNA Polymerase (20 U/μL)

Description

• Thermo Scientific™ Bacteriophage T7 RNA polymerase is DNA-dependent RNA polymerase with strict specificity its respective double-stranded promoter
• It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter
• Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides) Recommended for: Synthesis of unlabeled and labeled RNA that can be used:for hybridization, in vitro RNA translation; as aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing
• Note: Consensus promoter sequences: TAATACGACTCACTATA G GGAGA
• The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription
• Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.