Invitrogen™ T7 RNA Polymerase

Description

• Synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter
• A T7 RNA Polymerase technical bulletin is available
• Application: Synthesis of labeled and unlabeled RNA transcripts
• Source: Purified from E
• coli expressing the T7 RNA Polymerase gene on a plasmid Performance and Quality Testing: 3' and 5' exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction Unit Definition: One unit hydrolyzes 1nMol of ribonucleotide into acid-precipitable material in 1 hr at 37°C using a T7 transcription vector as template Unit Reaction Conditions: 40mM Tris-HCl (pH 8.0), 25mM NaCl, 8mM MgCl 2 , 2mM spermidine-(HCl) 3 , 5mM DTT, 0.4mM ATP, 0.4mM CTP, 0.4mM GTP, 0.4mM UTP, 1μCi [ 3H]GTP, 1μg Sph I-cut pT7L13, and enzyme in 50μL for 10 min
• at 37°C Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Wet Ice