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Poly(A) RNA Polymerase

SP6 RNA Polymerase

T3 RNA Polymerase

T7 RNA Polymerase

>200U/µL

1.25 U/μL

1X

20 U/μL

200 U/μL

200U/µL

20U/µL

5 U/μL

5X

1 kit

10,000 U

100 μL

2 x 2500 U

2 μL

20 μL

20,000 U

200 μL

2000 U

2000 Units

25 KU

25,000 U

2500 U

5 x 5000 U

5 x 5000 Units

5000 U

5000 Units

800 U

T7

200 U/μL T7 RNA Polymerase, 100 μLStore at -20°C.

5 U/μL Poly(A) Polymerase, 160 μLStore at -20°C.

Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2should be stored at –20°C.

Shipped on dry ice. Store at -20°C.

Store at –20°C.

Sufficient reagents for 5 G/I tailing, 20 reverse transcription, and 80 PCR reactions.HeLa Total RNA and Control Human Actin PCR Forward Primer to verify components and protocol.Store reagents at -15°C to -30°C in a non-frost-free freezer.Store HeLa Total RNA at -80°C.

T7 RNA Polymerase (50 U/μL) is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl2, 10 mM spermidine-(HCl)3, 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.

T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl2, 10 mM spermidine-(HCl)3, 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.