Invitrogen™Silencer™Select Negative Control No. 1 siRNA,in vivoready

Description

• Ambion™ Silencer ™ Select Negative Control siRNA, In Vivo Ready is extensively validated and an ideal control for many aspects of an siRNA experiment
• The control is highly purified as required for animal use
• 250 nmol is provided
• • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer ™ Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ™ Select siRNAs? Silencer ™ Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity
• The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data
• Silencer ™ Select GAPDH Positive Control siRNA: The Silencer ™ SelectGAPDH positive control is extensively validated and an ideal “test” siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines
• Because it targets GAPDH mRNA (a common internal control), many assays are available to measure its effects, including real-time RT-PCR as well as the Ambion™ KDalert™ GAPDH Assay Kit
• Quality Control: Silencer ™ Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards
• Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity
• Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly
• Furthermore, In Vivo Ready siRNAs undergo additional purification with a semipermeable membrane to remove excess salt
• This is followed by sterile filtratation and endotoxin testing
• At concentrations of 50 μM in deionized water, In Vivo Ready siRNAs contain
• less than 0.6 mM Na + , less than 2.0 mM K + , and less than 0.1 mM Mg 2+
• Silencer ™ Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer ™ Select siRNAs
• If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion™ Silencer ™ siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.