Invitrogen™ Ambion™mirVana™ miRNA Inhibitor, let-7c positive control

Description

• miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity
• We have now released the second generation of miRNA inhibitors, mir Vana™ miRNA Inhibitors, which: Display better potency than lead competitor's product Offer maximum and consistent effect in vitro at low miRNA inhibitor concentration Target the most updated Sanger content Can be used in vitro and in vivo , offering consistency throughout your entire research project Are non-toxic Positive and negative controls are also available for use along with mir Vana™ miRNA Inhibitors in miRNA down-regulation experiments
• mir Vana™ miRNA Inhibitor let-7c Positive Control The mir Vana™ miRNA Inhibitor let-7c Positive Control is intended for use as a positive control for mammalian cell culture experiments using mir Vana™ miRNA Inhibitors
• Endogenous let-7c miRNA has been shown to negatively regulate HMGA2 mRNA in cultured cells
• HMGA2 is a ubiquitously expressed, nonhistone, chromatin protein that can modulate gene expression through changes in chromatin architecture
• let-7c miRNA down-regulates levels of HMGA2 mRNA
• When transfected into human and mouse cell lines, let-7c miRNA inhibitor blocks endogenous let-7c miRNA, resulting in increased levels of HMGA2 mRNA
• Thus, let-7c miRNA inhibitor activity can be monitored in human or mouse cells using real-time reverse transcription PCR (RT-PCR) to detect HMGA2 mRNA
• First Generation Inhibitor Controls Our first-generation miRNA inhibitors (and controls), Ambion™ Anti-miR™ miRNA Inhibitors, have lower specificity and potency, plus a lower strand specificity compared to the new mir Vana™ miRNA Inhibitors
• These first-generation inhibitors are recommended for completing ongoing in vitro miRNA studies
• For new studies or in vivo studies, we highly recommend our new mir Vana™ miRNA Inhibitors and their corresponding controls.