Invitrogen™ Pre-miR™ miRNA Precursor Negative Control #1

Description

• Pre-miR™ miRNA Precursors are designed to mimic endogenous miRNAs
• Pre-miR miRNA Precursor Negative Control #1 is designed to use as a negative control for experiments using Pre-miR miRNA Precursors
• Transfect Pre-miR miRNA Precursor Negative Control #1 using the same methodology as for your experimental Pre-miR miRNA Precursors, then use the target gene expression from the sample transfected with the Pre-miR miRNA Precursor Negative Control #1 as a baseline for evaluation of the effect of the experimental Pre-miR miRNA Precursor on target gene expression
• Advantages of the Pre-miR™ miRNA Precursor Negative Control #1: Optimized design results in correct strand utilization by RISC Purified and ready to transfect About Pre-miR miRNA Precursors Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs
• This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs
• They enable detailed study of miRNA biological effects via gain-of-function experiments
• Using Pre-miR miRNA Precursor Negative Control #1 Pre-miR™ Negative Control #1 is a random sequence Pre-miR™ molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function
• RNA oligonucleotides are susceptible to degradation by exogenous ribonucleases introduced during handling
• Wear gloves when handling this product
• Use RNase-free reagents, tubes, and barrier pipette tips
• Upon receipt, store in a non-frost-free freezer at or below -20°C (dried oligonucleotides are shipped at ambient temperature)
• NOTE: Pre-miR™ miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.