Invitrogen™ Pre-miR™ hsa-miR-1 miRNA Precursor Postive Control

Description

• The Pre-miR™ hsa-miR-1 miRNA Precursor is intended for use as a positive control in experiments utilizing Pre-miR miRNA Precursors
• This precursor was chosen as a positive control because, upon delivery into cells, it effectively down regulates the expression of twinfilin-1, also known as PTK9, at the mRNA level (60-95% reduction
• Effective delivery and activity of the Pre-miR hsa-miR-1 miRNA Precursor can be detected by real-time PCR using a TaqMan™ Gene Expression Assay to PTK9 (Assay IDs: Hs00702289_s1 [human], Mm01598980_g1 [mouse])
• About Pre-miR miRNA Precursors Pre-miR™ miRNA Precursor Molecules are small, chemically modified double-stranded RNA molecules designed to mimic endogenous mature miRNAs
• These ready-to-use miRNA mimics, which are similar to, but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs
• They enable detailed study of miRNA biological effects via gain-of-function experiments (Figure 1)
• Positive and negative control Pre-miR miRNA Precursors (listed above) or the Pre-miR™ Precursor Starter Kit can be used to optimize transfection of Pre-miR miRNA Precursors
• Pre-miR miRNA Precursor Molecules have been carefully designed and modified to ensure that the correct strand, representing the desired mature miRNA, is taken up into the RNA-induced silencing-like complex responsible for miRNA activity (Figure 2)
• In contrast to miRNA expression vectors, these synthetic molecules can be used in dose response studies due to their introduction directly into the cell by transfection or electroporation
• Thanks to their small size, they are easier to transfect than vectors, and can be delivered using conditions identical to those used for siRNAs.